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Direct JKAP knockdown, <t>MK2206</t> and PD98059 treatment on RA Th17 differentiation (A–F) Western blot image examples (A), quantified p -AKT expression (B), quantified p -ERK expression (C) in RA CD4 + T cells among groups. IL-17A level (D) and CD4 + IL-17A + cell percentage (E-F) among groups. Data are represented as mean ± SEM. 6 samples in each group. Similar results were acquired from at least 3 independent experiments. ns: no significance; ∗: p < 0.05; ∗∗: p < 0.01; ∗∗∗: p < 0.001.
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Direct JKAP knockdown, <t>MK2206</t> and PD98059 treatment on RA Th17 differentiation (A–F) Western blot image examples (A), quantified p -AKT expression (B), quantified p -ERK expression (C) in RA CD4 + T cells among groups. IL-17A level (D) and CD4 + IL-17A + cell percentage (E-F) among groups. Data are represented as mean ± SEM. 6 samples in each group. Similar results were acquired from at least 3 independent experiments. ns: no significance; ∗: p < 0.05; ∗∗: p < 0.01; ∗∗∗: p < 0.001.
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Direct JKAP knockdown, MK2206 and PD98059 treatment on RA Th17 differentiation (A–F) Western blot image examples (A), quantified p -AKT expression (B), quantified p -ERK expression (C) in RA CD4 + T cells among groups. IL-17A level (D) and CD4 + IL-17A + cell percentage (E-F) among groups. Data are represented as mean ± SEM. 6 samples in each group. Similar results were acquired from at least 3 independent experiments. ns: no significance; ∗: p < 0.05; ∗∗: p < 0.01; ∗∗∗: p < 0.001.

Journal: iScience

Article Title: BMSC exosomes deliver JKAP to restore Th17/Treg balance via AKT/ERK, alleviating rheumatoid arthritis

doi: 10.1016/j.isci.2025.112832

Figure Lengend Snippet: Direct JKAP knockdown, MK2206 and PD98059 treatment on RA Th17 differentiation (A–F) Western blot image examples (A), quantified p -AKT expression (B), quantified p -ERK expression (C) in RA CD4 + T cells among groups. IL-17A level (D) and CD4 + IL-17A + cell percentage (E-F) among groups. Data are represented as mean ± SEM. 6 samples in each group. Similar results were acquired from at least 3 independent experiments. ns: no significance; ∗: p < 0.05; ∗∗: p < 0.01; ∗∗∗: p < 0.001.

Article Snippet: Cells were treated with 10 nM MK2206 (AKT inhibitor) or PD98059 (ERK inhibitor) (Selleck, USA) for 48 hours.

Techniques: Knockdown, Western Blot, Expressing

JKAP knockdown/MK2206/PD98059 stimulating CD4 + T cells on RA-FLS growth, invasion and inflammation (A–I) RA-FLS proliferation (A), TUNEL image examples (B), quantified apoptosis rate by TUNEL (C), Transwell image examples (D) among groups), quantified cell invasion by Transwell (E). Levels of IL-6 (F), IL-8 (G), CCL2, (H), MMP3 (I) among groups in RA-FLS supernatant. Data are represented as mean ± SEM. 10 samples in each group. Similar results were acquired from at least 3 independent experiments. For (B), it was captured at 5x lens, for (D), it was captured as 20x lens. ns: no significance; ∗: p < 0.05; ∗∗: p < 0.01.

Journal: iScience

Article Title: BMSC exosomes deliver JKAP to restore Th17/Treg balance via AKT/ERK, alleviating rheumatoid arthritis

doi: 10.1016/j.isci.2025.112832

Figure Lengend Snippet: JKAP knockdown/MK2206/PD98059 stimulating CD4 + T cells on RA-FLS growth, invasion and inflammation (A–I) RA-FLS proliferation (A), TUNEL image examples (B), quantified apoptosis rate by TUNEL (C), Transwell image examples (D) among groups), quantified cell invasion by Transwell (E). Levels of IL-6 (F), IL-8 (G), CCL2, (H), MMP3 (I) among groups in RA-FLS supernatant. Data are represented as mean ± SEM. 10 samples in each group. Similar results were acquired from at least 3 independent experiments. For (B), it was captured at 5x lens, for (D), it was captured as 20x lens. ns: no significance; ∗: p < 0.05; ∗∗: p < 0.01.

Article Snippet: Cells were treated with 10 nM MK2206 (AKT inhibitor) or PD98059 (ERK inhibitor) (Selleck, USA) for 48 hours.

Techniques: Knockdown, TUNEL Assay